Mass Spectrometry Lab

Overview of Equipment and Services

The mass spectrometry (MS) facility operates three high-resolution instruments: the Orbitrap Exploris (OE-240, Thermo, 2022), Q-Exactive (Thermo, 2013), and MALDI TOF-TOF (Bruker, 2016). Additionally, two low-resolution instruments are available: the LCMS2020 (Shimadzu, 2010) and GCMS2010S (Shimadzu, 2013). The OE-240 is primarily utilized for the analysis of digested and intact proteins using Nano and electrospray ionization (ESI) sources. The Q-Exactive employs soft ionization techniques, including ESI and atmospheric pressure chemical ionization (APCI), and is routinely used for accurate mass analysis of compounds within the m/z 100–3000 Da range. This instrument provides elemental analysis with a mass accuracy within 5ppm due to its high resolution. Open-access instruments, such as the MALDI, LCMS2020, and GCMS2010S, are always available to department users who have received prior training from facility personnel. The facility offers high- and low-resolution MS data for organic, bio-organic, intact protein, digested peptide, and oligomer analyses using ESI, MALDI, APCI, and electron ionization (EI) techniques.

Location and Hours of Operation

Primary: 450 Chevron Science Center, 219 Parkman Ave, Pittsburgh, PA, 15260

Primary Sq. Ft: 820

Days of the Week

Hours open

Monday – Friday

10:00 am – 6:00 pm

 

24/7 for trained users

Resources

Contacts

Name Role Phone Email Location
Bhaskar Godugu
Facility Director
 
336-554-4903
 
bhg5@pitt.edu
 
450 Chevron Science Center
 

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