ALERT

Upcoming Training Opportunities:

Do you need help finding specialized equipment or expert services for your research? Whether you are new to the Pitt research community or a regular shared facility user, join one of our upcoming webinars to learn more about the resources available across campus. These sessions will offer an overview of resources available, how to access equipment and services, iLab training, and an opportunity to ask questions.

Join one of the following two webinars:

No prior registration is required and anyone is welcome to join.

 

In addition, save the date for an in-person Shared Facility Showcase on the afternoon of Monday, October 19, 2026. More information about this event and how to register will be shared soon.

 

(hide this warning on this page)

Health Sciences Sequencing Core

Overview of Equipment and Services

The Health Sciences Sequencing Core (HSSC) is located on the 8th floor of the Rangos Research Building as part of the UPMC Children’s Hospital of Pittsburgh. Our facility offers next generation sequencing and spatial transcriptomics services to all University of Pittsburgh researchers. The HSSC operates two Illumina NextSeq 2000 instruments with Illumina DRAGEN servers that can generate up to 500Gb of sequence data per flow cell, and one MiSeq instrument for targeted resequencing, small genome, metagenomic and microbiome studies. For sample QC, our core utilizes a Fragment Analyzer (Agilent), Qubit Fluorometers (Invitrogen) and a TapeStation (Agilent). For spatial transcriptomics services, we offer end-to-end support for 10x Visium and 10x Xenium workflows. We operate two 10x Genomics CytAssist for Visium and Visium HD workflows, and one 10x Genomics Xenium Analyzer for running in situ Xenium workflows.

Location and Hours of Operation

Primary: Rangos Research Center-8145, 530 45th St, Pittsburgh, PA, 15201

Primary Sq. Ft: 1350

Days of the Week

Hours open

Monday – Friday

9:00 am – 5:00 pm

Resources

Contacts

Name Role Phone Email Location
William MacDonald, PhD
Associate Director (Facility Director)
 

 
wam30@pitt.edu
 
Rangos Research Center 8145
 
Amanda C. Poholek, PhD
Faculty Director
 

 
poholeka@pitt.edu
 
The Assembly Building
 
HSSC General Email

 

 
hsscchp@pitt.edu
 

 
Rania Elbakri, MSc
Senior Research Staff
 

 
rania.elbakri@chp.edu
 
Rangos Research Center 8145
 
Yvette Rhodes
Senior Research Staff
 

 
ylr3@pitt.edu
 
Rangos Research Center 8145
 
Bryan Thompson
Senior Research Staff
 

 
brt2@pitt.edu
 
Rangos Research Center 8145
 
Yvonne Eisele, PhD
Senior Research Staff
 

 
eiseley@pitt.edu
 
Rangos Research Center 8145
 

Map