Cytometry Facility

Getting Started

To request services, you need register for iLab.

Users with UPitt IDs

Please register for iLab by clicking HERE

Users without a UPitt ID, register for an iLab account:

Please click HERE to fill out the required information and request an account.  You will receive a password within one business day.

Registered Users:

If you have an iLab account, log in at the top right of this page.

1) To reserve time on the equipment, click on the Schedule Equipment tab.  View the calendar by pressing the View Schedule button.  Users who require training, please press the Request Training button. 

2) To request a service, click on the Request Service tab.  At the top is a list of service categories. Click on the category and press the Request Service button to initiate the request.  Complete the form and submit to the core. 

Have an account but need a password?

If you have an iLab account, but you do not know your password, you may request a new password.  Click HERE to create a new password.

Overview of Services

Cytometry can rapidly provide quantitative information regarding a wide variety of cellular properties, including DNA content; expression of surface, cytoplasmic, and nuclear antigens; receptor-ligand interactions; chromosome analysis; phagocytosis; target-effector cell binding; ion fluxes; expression of genes or gene products; and various light scattering properties. Thus, flow cytometry is extremely useful for a broad range of basic research projects as well as clinical monitoring studies.

The mission of the UPCI Cytometry Facility (CF) is to:

• Train investigators, their students and staff to use analytical cytometry to its fullest advantage in their research;
  • Basic and advanced training in the use of all facility cytometers is offered continuously.
  • Facility is available to trained users 24 hours a day, seven days a week.
  • High speed workstations are equipped with a selection of cytometry analysis programs.
• Provide high quality cell separation by fluorescence activated cell sorting (FACS);
  • Facility is operated as a BSL-2+ facility.
  • Can sort live human cells and cells transfected with retroviral constructs.
• Consult on experimental design, analysis and assay development, which is available to UPCI investigators without charge.
• Digital Spatial Profiling
• Protein: nCounter
• RNA: GeoMx
• Single Cell: CosMX

Leadership

Tullia Bruno, PhD
Director, Cytometry Facility
Phone: 412-623-1780
Email: tbruno@pitt.edu

Location and hours of operation

Contacts